Structural and electrostatic characterization of pariacoto virus: implications for viral assembly.

ABSTRACT: We present the first all-atom model for the structure of a T = 3 virus, pariacoto virus (PaV), which is a nonenveloped, icosahedral RNA virus and a member of the Nodaviridae family. The model is an extension of the crystal structure, which reveals about 88% of the protein structure but only about 35% of the RNA structure. New modeling methods, combining coarse-grained and all-atom approaches, were required for developing the model. Evaluation of alternative models confirms our earlier observation that the polycationic N- and C-terminal tails of the capsid proteins must penetrate deeply into the core of the virus, where they stabilize the structure by neutralizing a substantial fraction of the RNA charge. This leads us to propose a model for the assembly of small icosahedral RNA viruses: no

SUBMITTER: Devkota B 

PROVIDER: S-EPMC2720792 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature