Morphological features and signature gene response elicited by inactivation of FtsI in Mycobacterium tuberculosis.

ABSTRACT:

Objectives

Universally conserved events in cell division provide the opportunity for the development of novel chemotherapeutics against Mycobacterium tuberculosis. The aim of this study was to use the beta-lactam antimicrobials cefalexin and piperacillin to inhibit FtsI and characterize the morphological changes and global transcriptional activities of genes to identify a signature response to FtsI inactivation.

Methods

Cefalexin and piperacillin were used to block cell division, and microscopy was used to evaluate the effects on bacterial morphology and ultrastructure. Global transcriptional analysis was performed to determine the impact of FtsI inhibition on cell cycle processes and to identify molecular markers.

Results

Inhibition of FtsI with cefalexin and piperac

SUBMITTER: Slayden RA 

PROVIDER: S-EPMC2721702 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature