Selection of reference genes for normalisation of real-time RT-PCR in brain-stem death injury in Ovis aries.

ABSTRACT:

Background

Heart and lung transplantation is frequently the only therapeutic option for patients with end stage cardio respiratory disease. Organ donation post brain stem death (BSD) is a pre-requisite, yet BSD itself causes such severe damage that many organs offered for donation are unusable, with lung being the organ most affected by BSD. In Australia and New Zealand, less than 50% of lungs offered for donation post BSD are suitable for transplantation, as compared with over 90% of kidneys, resulting in patients dying for lack of suitable lungs. Our group has developed a novel 24 h sheep BSD model to mimic the physiological milieu of the typical human organ donor. Characterisation of the gene expression changes associated with BSD is critical and will assist in determining the a

SUBMITTER: Passmore M 

PROVIDER: S-EPMC2721835 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature