Identifying the topology of protein complexes from affinity purification assays.

ABSTRACT:

Motivation

Recent advances in high-throughput technologies have made it possible to investigate not only individual protein interactions, but also the association of these proteins in complexes. So far the focus has been on the prediction of complexes as sets of proteins from the experimental results. The modular substructure and the physical interactions within the protein complexes have been mostly ignored.

Results

We present an approach for identifying the direct physical interactions and the subcomponent structure of protein complexes predicted from affinity purification assays. Our algorithm calculates the union of all maximum spanning trees from scoring networks for each protein complex to extract relevant interactions. In a subsequent step this network is extended to

SUBMITTER: Friedel CC 

PROVIDER: S-EPMC2723003 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature