Rare codon content affects the solubility of recombinant proteins in a codon bias-adjusted Escherichia coli strain.

ABSTRACT:

Background

The expression of heterologous proteins in Escherichia coli is strongly affected by codon bias. This phenomenon occurs when the codon usage of the mRNA coding for the foreign protein differs from that of the bacterium. The ribosome pauses upon encountering a rare codon and may detach from the mRNA, thereby the yield of protein expression is reduced. Several bacterial strains have been engineered to overcome this effect. However, the increased rate of translation may lead to protein misfolding and insolubilization. In order to prove this assumption, the solubility of several recombinant proteins from plants was studied in a codon bias-adjusted E. coli strain.

Results

The expression of eight plant proteins in Escherichia coli BL21(DE3)-pLysS and BL21(DE3)-CodonPlus-

SUBMITTER: Rosano GL 

PROVIDER: S-EPMC2723077 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature