Functional analysis of the C-reactive protein (CRP) gene -717A>G polymorphism associated with coronary heart disease.

ABSTRACT:

Background

Atherosclerosis underlies the major pathophysiological mechanisms of coronary heart disease (CHD), and inflammation contributes to all phases of atherosclerosis. C-reactive protein (CRP), a sensitive, but nonspecific marker of inflammation has been shown to play proatherogenic roles in the process of atherosclerosis. Our previous report showed that rs2794521 (-717A>G), located in the promoter of the CRP gene, was independently associated with CHD in Chinese subjects. In the present study, we tried to investigate the biological significance of this genetic variation in vitro.

Methods

The influence of G to A substitution at the site of rs2794521 on the transcriptional activity of the promoter of the CRP gene was assessed by luciferase reporter assay, and protein bin

SUBMITTER: Wang L 

PROVIDER: S-EPMC2723087 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature