Quantitative proteomics of nutrient limitation in the hydrogenotrophic methanogen Methanococcus maripaludis.

ABSTRACT:

Background

Methanogenic Archaea play key metabolic roles in anaerobic ecosystems, where they use H2 and other substrates to produce methane. Methanococcus maripaludis is a model for studies of the global response to nutrient limitations.

Results

We used high-coverage quantitative proteomics to determine the response of M. maripaludis to growth-limiting levels of H2, nitrogen, and phosphate. Six to ten percent of the proteome changed significantly with each nutrient limitation. H2 limitation increased the abundance of a wide variety of proteins involved in methanogenesis. However, one protein involved in methanogenesis decreased: a low-affinity [Fe] hydrogenase, which may dominate over a higher-affinity mechanism when H2 is abundant. Nitrogen limitation increased known nitrog

SUBMITTER: Xia Q 

PROVIDER: S-EPMC2723118 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature