Role of kinesin-1 and cytoplasmic dynein in endoplasmic reticulum movement in VERO cells.

ABSTRACT: Generating the extended endoplasmic reticulum (ER) network depends on microtubules, which act as tracks for motor-driven ER tubule movement, generate the force to extend ER tubules by means of attachment to growing microtubule plus-ends and provide static attachment points. We have analysed ER dynamics in living VERO cells and find that most ER tubule extension is driven by microtubule motors. Surprisingly, we observe that approximately 50% of rapid ER tubule movements occur in the direction of the centre of the cell, driven by cytoplasmic dynein. Inhibition of this movement leads to an accumulation of lamellar ER in the cell periphery. By expressing dominant-negative kinesin-1 constructs, we show that kinesin-1 drives ER tubule extension towards the cell periphery and that this motility i

SUBMITTER: Wozniak MJ 

PROVIDER: S-EPMC2723153 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature