Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus.

ABSTRACT: In this article, a panel of ssDNA aptamers specific to Staphylococcus aureus was obtained by a whole bacterium-based SELEX procedure and applied to probing S. aureus. After several rounds of selection with S. aureus as the target and Streptococcus and S. epidermidis as counter targets, the highly enriched oligonucleic acid pool was sequenced and then grouped under different families on the basis of the homology of the primary sequence and the similarity of the secondary structure. Eleven sequences from different families were selected for further characterization by confocal imaging and flow cytometry analysis. Results showed that five aptamers demonstrated high specificity and affinity to S. aureus individually. The five aptamers recognize different molecular targets by competitive experi

SUBMITTER: Cao X 

PROVIDER: S-EPMC2724295 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature