Anchoring fusion thrombomodulin to the endothelial lumen protects against injury-induced lung thrombosis and inflammation.

ABSTRACT:

Rationale

Endothelial thrombomodulin (TM) regulates thrombosis and inflammation. Diverse forms of pulmonary and vascular injury are accompanied by down-regulation of TM, which aggravates tissue injury. We postulated that anchoring TM to the endothelial surface would restore its protective functions.

Objectives

To design an effective and safe strategy to treat pulmonary thrombotic and inflammatory injury.

Methods

We synthesized a fusion protein, designated scFv/TM, by linking the extracellular domain of mouse TM to a single-chain variable fragment of an antibody to platelet endothelial cell adhesion molecule-1 (PECAM-1). The targeting and protective functions of scFv/TM were tested in mouse models of lung ischemia-reperfusion and acute lung injury (ALI) caused by intra

SUBMITTER: Ding BS 

PROVIDER: S-EPMC2724717 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature