Regulation of Rap1 activity is required for differential adhesion, cell-type patterning and morphogenesis in Dictyostelium.

ABSTRACT: Regulated cell adhesion and motility have important roles during growth, development and tissue homeostasis. Consequently, great efforts have been made to identify genes that control these processes. One candidate is Rap1, as it has been implicated in the regulation of adhesion and motility in cell culture. To further study the role of Rap1 during multicellular development, we generated a mutant in a potential Rap1 GTPase activating protein (RapGAPB) in Dictyostelium. rapGAPB(-) cells have increased levels of active Rap1 compared with wild-type cells, indicating that RapGAPB regulates Rap1 activity. Furthermore, rapGAPB(-) cells exhibit hallmark phenotypes of other known mutants with hyperactivated Rap1, including increased substrate adhesion and abnormal F-actin distribution. However, unl

SUBMITTER: Parkinson K 

PROVIDER: S-EPMC2724730 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature