Combinatorial probabilistic chromatin interactions produce transcriptional heterogeneity.

ABSTRACT: Gene regulation often appears deterministic in the average cell population, but transcription is a probabilistic process at the single-cell level. Although many mechanisms are invoked to account for this behavior, it is difficult to determine how cell-to-cell variation in the interactions of transcription factors with target chromatin impact transcriptional output. Here, we use cells that contain a 200-copy tandem array of promoter or reporter gene units to simultaneously visualize transient interaction, equilibrium or steady-state binding of fluorescent-protein-labeled glucocorticoid receptor with its DNA response elements, the recruitment of diverse coregulators, and transcriptional output at the single-cell level. These regulatory proteins associate with target chromatin via a probabili

SUBMITTER: Voss TC 

PROVIDER: S-EPMC2724732 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature