Plasmid-based system for high-level gene expression and antisense gene knockdown in Bartonella henselae.

ABSTRACT: Six broad-host-range plasmid vectors were developed to study gene expression in Bartonella henselae. The vectors were used to express a beta-galactosidase reporter gene in B. henselae and to generate antisense RNA for gene knockdown. When applied to ompR, a putative transcription response regulator of B. henselae, this antisense RNA gene knockdown strategy reduced bacterial invasion of human endothelial cells by over 60%.

SUBMITTER: Gillaspie D 

PROVIDER: S-EPMC2725459 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature