Transcriptional profiling of methyltransferase genes during growth of Methanosarcina mazei on trimethylamine.

ABSTRACT: The genomic expression patterns of Methanosarcina mazei growing with trimethylamine were measured in comparison to those of cells grown with methanol. We identified a total of 72 genes with either an increased level (49 genes) or a decreased level (23 genes) of mRNA during growth on trimethylamine with methanol-grown cells as the control. Major differences in transcript levels were observed for the mta, mtb, mtt, and mtm genes, which encode enzymes involved in methane formation from methanol and trimethylamine, respectively. Other differences in mRNA abundance were found for genes encoding enzymes involved in isopentenyl pyrophosphate synthesis and in the formation of aromatic amino acids, as well as a number of proteins with unknown functions. The results were verified by in-depth analysi

SUBMITTER: Kratzer C 

PROVIDER: S-EPMC2725588 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature