Hydroxyl radicals are involved in cell killing by the bacterial topoisomerase I cleavage complex.

ABSTRACT: Escherichia coli expressing SOS-inducing mutant topoisomerase I was utilized to demonstrate that covalent protein-DNA complex accumulation results in oxidative damage. Hydroxyl radicals were detected following mutant topoisomerase induction. The presence of the Fe(2+) chelator 2,2'-dipyridyl and an iscS mutation affecting Fe-S cluster formation protect against topoisomerase I cleavage complex-mediated cell killing.

SUBMITTER: Liu IF 

PROVIDER: S-EPMC2725605 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature