Inaccuracy of single-target sequencing for discriminating species of the Mycobacterium abscessus group.
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ABSTRACT:
SUBMITTER: Macheras E
PROVIDER: S-EPMC2725694 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
Publications
Inaccuracy of single-target sequencing for discriminating species of the Mycobacterium abscessus group.
Macheras Edouard E Roux Anne-Laure AL Ripoll Fabienne F Sivadon-Tardy Valérie V Gutierrez Cristina C Gaillard Jean-Louis JL Heym Beate B
Journal of clinical microbiology 20090610 8
We determined nucleotide sequences of rpoB, hsp65, and sodA in 59 clinical isolates (from 58 patients) of the Mycobacterium abscessus group. Identification to the species level, based on three target genes, was concordant for 44 isolates (25 M. abscessus, 13 Mycobacterium massiliense, and 6 Mycobacterium bolletii isolates) and discordant for 15 isolates which had "interspecific composite patterns." Sequence analysis of five housekeeping genes also showed composite patterns in 8 of these 15 isola ...[more]
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Project description:Treatment efficacy of Mycobacterium abscessus infections depends on bacterial genotype. Here, the relationship between genotype, as determined by sequence analysis, and antibiotic resistance phenotype was analyzed. The results demonstrate that M. abscessus genotype characteristics, including erm(41) sequevar and mutations of rrl and rrs, are predictive of clarithromycin and amikacin resistance.
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