Spatio-temporal dynamics of replication and transcription sites in the mammalian cell nucleus.

ABSTRACT: To study when and where active genes replicated in early S phase are transcribed, a series of pulse-chase experiments are performed to label replicating chromatin domains (RS) in early S phase and subsequently transcription sites (TS) after chase periods of 0 to 24 h. Surprisingly, transcription activity throughout these chase periods did not show significant colocalization with early RS chromatin domains. Application of novel image segmentation and proximity algorithms, however, revealed close proximity of TS with the labeled chromatin domains independent of chase time. In addition, RNA polymerase II was highly proximal and showed significant colocalization with both TS and the chromatin domains. Based on these findings, we propose that chromatin activated for transcription dynamically un

SUBMITTER: Malyavantham KS 

PROVIDER: S-EPMC2725786 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature