Effects of S1 cleavage on the structure, surface export, and signaling activity of human Notch1 and Notch2.

ABSTRACT:

Background

Notch receptors are normally cleaved during maturation by a furin-like protease at an extracellular site termed S1, creating a heterodimer of non-covalently associated subunits. The S1 site lies within a key negative regulatory region (NRR) of the receptor, which contains three highly conserved Lin12/Notch repeats and a heterodimerization domain (HD) that interact to prevent premature signaling in the absence of ligands. Because the role of S1 cleavage in Notch signaling remains unresolved, we investigated the effect of S1 cleavage on the structure, surface trafficking and ligand-mediated activation of human Notch1 and Notch2, as well as on ligand-independent activation of Notch1 by mutations found in human leukemia.

Principal findings

The X-ray structure of the N

SUBMITTER: Gordon WR 

PROVIDER: S-EPMC2726630 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature