The CCR4-NOT complex physically and functionally interacts with TRAMP and the nuclear exosome.

ABSTRACT:

Background

Ccr4-Not is a highly conserved multi-protein complex consisting in yeast of 9 subunits, including Not5 and the major yeast deadenylase Ccr4. It has been connected functionally in the nucleus to transcription by RNA polymerase II and in the cytoplasm to mRNA degradation. However, there has been no evidence so far that this complex is important for RNA degradation in the nucleus.

Methodology/principal findings

In this work we point to a new role for the Ccr4-Not complex in nuclear RNA metabolism. We determine the importance of the Ccr4-Not complex for the levels of non-coding nuclear RNAs, such as mis-processed and polyadenylated snoRNAs, whose turnover depends upon the nuclear exosome and TRAMP. Consistently, mutation of both the Ccr4-Not complex and the nuclear ex

SUBMITTER: Azzouz N 

PROVIDER: S-EPMC2727002 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature