In vivo imaging, tracking, and targeting of cancer stem cells.

ABSTRACT:

Background

There is increasing evidence that solid cancers contain cancer-initiating cells (CICs) that are capable of regenerating a tumor that has been surgically removed and/or treated with chemotherapy and/or radiation therapy. Currently, cell surface markers, like CD133 or CD44, are used to identify CICs in vitro; however, these markers cannot be used to identify and track CICs in vivo. The 26S proteasome is the main regulator of many processes within a proliferating cell, and its activity may be altered depending on the phenotype of a cell.

Methods

Human glioma and breast cancer cells were engineered to stably express ZsGreen fused to the carboxyl-terminal degron of ornithine decarboxylase, resulting in a fluorescent fusion protein that accumulates in cells in the absen

SUBMITTER: Vlashi E 

PROVIDER: S-EPMC2727141 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature