Progenitors of skeletal muscle satellite cells express the muscle determination gene, MyoD.

ABSTRACT: Satellite cells are tissue-specific stem cells responsible for skeletal muscle growth and regeneration. Although satellite cells were identified almost 50 years ago, the identity of progenitor populations from which they derive remains controversial. We developed MyoD(iCre) knockin mice, and used Cre/lox lineage analysis to determine whether satellite cell progenitors express MyoD, a marker of myogenic commitment. Recombination status of satellite cells was determined by confocal microscopy of isolated muscle fibers and by electron microscopic observation of muscle tissue fixed immediately following isolation, using R26R-EYFP and R26R (beta-gal) reporter mice, respectively. We show that essentially all adult satellite cells associated with limb and body wall musculature, as well as the dia

SUBMITTER: Kanisicak O 

PROVIDER: S-EPMC2728477 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature