Zebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine.

ABSTRACT:

Background

Addiction is a pathological dysregulation of the brain's reward systems, determined by several complex genetic pathways. The conditioned place preference test provides an evaluation of the effects of drugs in animal models, allowing the investigation of substances at a biologically relevant level with respect to reward. Our lab has previously reported the development of a reliable conditioned place preference paradigm for zebrafish. Here, this test was used to isolate a dominant N-ethyl-N-nitrosourea (ENU)-induced mutant, no addiction (nad(dne3256)), which fails to respond to amphetamine, and which we used as an entry point towards identifying the behaviorally relevant transcriptional response to amphetamine.

Results

Through the combination of microarray experimen

SUBMITTER: Webb KJ 

PROVIDER: S-EPMC2728535 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature