Do sex steroids exert sex-specific and/or opposite effects on gene expression in lacrimal and meibomian glands?

ABSTRACT:

Purpose

We hypothesize that sex steroids induce sex-specific and/or opposite effects in the lacrimal and meibomian glands and that these actions may influence the prevalence of dry eye syndrome. The objective of this study was to begin to test this hypothesis.

Methods

Lacrimal and meibomian glands were obtained from ovariectomized mice that had been treated with testosterone or control vehicle for 14 days. Samples were processed for the isolation of RNA, and analyzed for differentially expressed mRNAs using CodeLink Bioarrays and quantitative real-time PCR (qPCR) techniques. Data were compared to those obtained following testosterone treatment of orchiectomized mice, as well as after the administration of 17beta-estradiol and/or progesterone to ovariectomized mice.

Result

SUBMITTER: Sullivan DA 

PROVIDER: S-EPMC2728565 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature