Correlations between RNA and protein expression profiles in 23 human cell lines.

ABSTRACT:

Background

The Central Dogma of biology holds, in famously simplified terms, that DNA makes RNA makes proteins, but there is considerable uncertainty regarding the general, genome-wide correlation between levels of RNA and corresponding proteins. Therefore, to assess degrees of this correlation we compared the RNA profiles (determined using both cDNA- and oligo-based microarrays) and protein profiles (determined immunohistochemically in tissue microarrays) of 1066 gene products in 23 human cell lines.

Results

A high mean correlation coefficient (0.52) was obtained from the pairwise comparison of RNA levels determined by the two platforms. Significant correlations, with correlation coefficients exceeding 0.445, between protein and RNA levels were also obtained for a third of

SUBMITTER: Gry M 

PROVIDER: S-EPMC2728742 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature