Characterization of novel VP7, VP4, and VP6 genotypes of a previously untypeable group A rotavirus.

ABSTRACT: Rotavirus is the most common cause of acute gastroenteritis among infants and young children throughout the world, but rotavirus cases in developing countries account for nearly all of the approximately 600,000 annual deaths. We studied the epidemiology of rotavirus in 22 rural communities in northern coastal Ecuador over a five-year period. From 250 rotavirus positive stool specimens, the percentage that could not be RT-PCR genotyped for VP4 and VP7 was 77% and 63%, respectively. The possibility of sample degradation was considered but discounted after an experimental examination of rotavirus stability and EM visualization of rotavirus-like particles in several untypeable samples. Finally, alternate primers were used to amplify Ecu534, a sample that was untypeable using most published VP4

SUBMITTER: Solberg OD 

PROVIDER: S-EPMC2729439 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature