Characterisation of the effect of a simulated hydrocarbon spill on diazotrophs in mangrove sediment mesocosm.

ABSTRACT: An analysis of the effect of an oil spill on mangrove sediments was carried out by contamination of mesocosms derived from two different mangroves, one with a history of contamination and one pristine. The association between N(2) fixers and hydrocarbon degradation was assessed using quantitative PCR (qPCR) for the genes rrs and nifH, nifH clone library sequencing and total petroleum hydrocarbon (TPH) quantification using gas chromatography. TPH showed that the microbial communities of both mangroves were able to degrade the hydrocarbons added; however, whereas the majority of oil added to the mesocosm derived from the polluted mangrove was degraded in the 75 days of the experiment, there was only partially degradation in the mesocosm derived from the pristine mangrove. qPCR showed that th

SUBMITTER: Taketani RG 

PROVIDER: S-EPMC2729449 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature