A chromatin insulator mediates transgene homing and very long-range enhancer-promoter communication.

ABSTRACT: Insulator sequences help to organize the genome into discrete functional regions by preventing inappropriate cross-regulation. This is thought to be mediated in part through associations with other insulators located elsewhere in the genome. Enhancers that normally drive Drosophila even skipped (eve) expression are located closer to the TER94 transcription start site than to that of eve. We discovered that the region between these genes has enhancer-blocking activity, and that this insulator region also mediates homing of P-element transgenes to the eve-TER94 genomic neighborhood. Localization of these activities to within 0.6 kb failed to separate them. Importantly, homed transgenic promoters respond to endogenous eve enhancers from great distances, and this long-range communication depen

SUBMITTER: Fujioka M 

PROVIDER: S-EPMC2730365 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature