Morpholino monolayers: preparation and label-free DNA analysis by surface hybridization.

ABSTRACT: Surface hybridization, a reaction in which nucleic acid molecules in solution react with nucleic acid partners immobilized on a surface, is widely practiced in life science research. In these applications the immobilized partner, or "probe", is typically single-stranded DNA. Because DNA is strongly charged, high salt conditions are required to enable binding between analyte nucleic acids ("targets") in solution and the DNA probes. High salt, however, compromises prospects for label-free monitoring or control of the hybridization reaction through surface electric fields; it also stabilizes secondary structure in target species that can interfere with probe-target recognition. In this work, initial steps toward addressing these challenges are taken by introducing morpholinos, a class of unch

SUBMITTER: Tercero N 

PROVIDER: S-EPMC2730437 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature