Aberrant cell cycle progression and endoreplication in regenerating livers of mice that lack a single E-type cyclin.

ABSTRACT:

Background & aims

E-cyclins control the transition of quiescent cells into the cell cycle. Two E-cyclins, CcnE1 and CcnE2, have been described, but their specific contributions to cell cycle reentry in vivo are poorly understood. Liver regeneration following partial hepatectomy is an excellent in vivo model for the study of cell cycle reentry of quiescent cells. We investigated the relevance of E-cyclins in directing resting hepatocytes into the cell cycle after partial hepatectomy using CcnE1 and CcnE2 knockout mice.

Methods

Partial hepatectomy (70%) was performed in CcnE1 (E1(-/-)) and CcnE2 (E2(-/-)) knockout and wild-type mice. Liver regeneration was monitored by cell cycle markers for G(1)/S phase, S phase, and M phase as well as by determining the liver/body weight rat

SUBMITTER: Nevzorova YA 

PROVIDER: S-EPMC2730664 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature