Microarrays with varying carbohydrate density reveal distinct subpopulations of serum antibodies.

ABSTRACT: Antigen arrays have become important tools for profiling complex mixtures of proteins such as serum antibodies. These arrays can be used to better understand immune responses, discover new biomarkers, and guide the development of vaccines. Nevertheless, they are not perfect and improved array designs would enhance the information derived from this technology. In this study, we describe and evaluate a strategy for varying antigen density on an array and then use the array to study binding of lectins, monoclonal antibodies, and serum antibodies. To vary density, neoglycoproteins containing differing amounts of carbohydrate were synthesized and used to make a carbohydrate microarray with variations in both structure and density. We demonstrate that this method provides variations in density o

SUBMITTER: Oyelaran O 

PROVIDER: S-EPMC2730745 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature