The SWI/SNF chromatin remodeling complex regulates myocardin-induced smooth muscle-specific gene expression.

ABSTRACT:

Objective

Regulatory complexes comprising myocardin and serum response factor (SRF) are critical for the transcriptional regulation of many smooth muscle-specific genes. However, little is known about the epigenetic mechanisms that regulate the activity of these complexes. In the current study, we investigated the role of SWI/SNF ATP-dependent chromatin remodeling enzymes in regulating the myogenic activity of myocardin.

Methods and results

We found that both Brg1 and Brm are required for maintaining expression of several smooth muscle-specific genes in primary cultures of aortic smooth muscle cells. Furthermore, the ability of myocardin to induce expression of smooth muscle-specific genes is abrogated in cells expressing dominant negative Brg1. In SW13 cells, which lack end

SUBMITTER: Zhou J 

PROVIDER: S-EPMC2730881 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature