High-throughput retrotransposon-based fluorescent markers: improved information content and allele discrimination.

ABSTRACT:

Background

Dense genetic maps, together with the efficiency and accuracy of their construction, are integral to genetic studies and marker assisted selection for plant breeding. High-throughput multiplex markers that are robust and reproducible can contribute to both efficiency and accuracy. Multiplex markers are often dominant and so have low information content, this coupled with the pressure to find alternatives to radio-labelling, has led us to adapt the SSAP (sequence specific amplified polymorphism) marker method from a 33P labelling procedure to fluorescently tagged markers analysed from an automated ABI 3730 xl platform. This method is illustrated for multiplexed SSAP markers based on retrotransposon insertions of pea and is applicable for the rapid and efficient generation

SUBMITTER: Knox M 

PROVIDER: S-EPMC2731089 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature