Negative regulators of insulin signaling revealed in a genome-wide functional screen.

ABSTRACT:

Background

Type 2 diabetes develops due to a combination of insulin resistance and beta-cell failure and current therapeutics aim at both of these underlying causes. Several negative regulators of insulin signaling are known and are the subject of drug discovery efforts. We sought to identify novel contributors to insulin resistance and hence potentially novel targets for therapeutic intervention.

Methodology

An arrayed cDNA library encoding 18,441 human transcripts was screened for inhibitors of insulin signaling and revealed known inhibitors and numerous potential novel regulators. The novel hits included proteins of various functional classes such as kinases, phosphatases, transcription factors, and GTPase associated proteins. A series of secondary assays confirmed the re

SUBMITTER: Huang SM 

PROVIDER: S-EPMC2731165 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature