Activated integrin VLA-4 localizes to the lamellipodia and mediates T cell migration on VCAM-1.

ABSTRACT: Lymphocyte migration from blood into lymphoid tissues or to sites of inflammation occurs through interactions between cell surface integrins and their ligands expressed on the vascular endothelium and the extracellular matrix. VLA-4 (alpha(4)beta(1)) is a key integrin in the effective trafficking of lymphocytes. Although it has been well established that integrins undergo functionally significant conformational changes to mediate cell adhesion, there is no mechanistic information that explains how these are dynamically and spatially regulated during lymphocyte polarization and migration. Using dynamic fluorescence resonance energy transfer analysis of a novel VLA-4 FRET sensor under total internal reflection fluorescence microscopy, we show that VLA-4 activation localizes to the lamellipod

SUBMITTER: Hyun YM 

PROVIDER: S-EPMC2731303 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature