Proteomic profiling of nonenzymatically glycated proteins in human plasma and erythrocyte membranes.

ABSTRACT: Nonenzymatic glycation of peptides and proteins by d-glucose has important implications in the pathogenesis of diabetes mellitus, particularly in the development of diabetic complications. In this work, we report the first proteomics-based characterization of nonenzymatically glycated proteins in human plasma and erythrocyte membranes from individuals with normal glucose tolerance, impaired glucose tolerance, and type 2 diabetes mellitus. Phenylboronate affinity chromatography was used to enrich glycated proteins and glycated tryptic peptides from both human plasma and erythrocyte membranes. The enriched peptides were subsequently analyzed by liquid chromatography coupled with electron transfer dissociation-tandem mass spectrometry, resulting in the confident identification of 76 and 31 pr

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC2731429 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature