Protein phosphatase 1 regulates exit from the spindle checkpoint in budding yeast.

ABSTRACT: Accurate chromosome segregation depends on sister kinetochores coming under tension when they make bioriented attachments to microtubules from opposite poles. The spindle checkpoint halts the cell cycle in response to defects in generating proper attachments or tension on kinetochores, although the precise signal that triggers the checkpoint is unclear because tension and attachment are coupled. The target of the checkpoint is the Cdc20 protein, which initiates the anaphase-promoting complex (APC)-dependent degradation of the anaphase inhibitor Pds1/securin. Although the molecular details of spindle checkpoint activation are still being elucidated, phosphorylation by at least four kinases is a crucial requirement. However, less is known about the mechanisms that silence the checkpoint afte

SUBMITTER: Pinsky BA 

PROVIDER: S-EPMC2731492 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature