Antibody-based screen for ionizing radiation-dependent changes in the Mammalian proteome for use in biodosimetry.

ABSTRACT: In an effort to identify proteomic changes that may be useful for radiation biodosimetry, human cells of hematological origin were treated with ionizing radiation or mock-irradiated and then harvested at different times after treatment. Protein lysates were generated from these cells and evaluated by Western blotting using a panel of 301 commercially available antibodies targeting 161 unique proteins. From this screen, we identified 55 ionizing radiation-responsive proteins, including 14 proteins not previously reported to be radiation-responsive at the protein level. The data from this large-scale screen have been assembled into a public website ( http://labs.fhcrc.org/paulovich/biodose_index.html ) that may be of value to the radiation community both as a source of putative biomarkers fo

SUBMITTER: Ivey RG 

PROVIDER: S-EPMC2731583 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature