Gas6-axl receptor signaling is regulated by glucose in vascular smooth muscle cells.

ABSTRACT:

Objective

The receptor tyrosine kinase Axl and its ligand Gas6 are involved in the development of renal diabetic disease. In vascular smooth muscle cells (VSMCs) Axl is activated by reactive oxygen species and stimulates migration and cell survival, suggesting a role for Axl in the vascular complications of diabetes.

Methods and results

We investigated the effect of varying glucose concentration on Axl signaling in VSMCs. Glucose exerted powerful effects on Gas6-Axl signaling with greater activation of Akt and mTOR in low glucose, and greater activation of ERK1/2 in high glucose. Plasma membrane distribution and tyrosine phosphorylation of Axl were not affected by glucose. However, coimmunoprecipitation studies demonstrated that glucose changed the interaction of Axl with it

SUBMITTER: Cavet ME 

PROVIDER: S-EPMC2731942 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature