Relationships between circulating metabolic intermediates and insulin action in overweight to obese, inactive men and women.

ABSTRACT:

Objective

To determine whether circulating metabolic intermediates are related to insulin resistance and beta-cell dysfunction in individuals at risk for type 2 diabetes.

Research design and methods

In 73 sedentary, overweight to obese, dyslipidemic individuals, insulin action was derived from a frequently sampled intravenous glucose tolerance test. Plasma concentrations of 75 amino acids, acylcarnitines, free fatty acids, and conventional metabolites were measured with a targeted, mass spectrometry-based platform. Principal components analysis followed by backward stepwise linear regression was used to explore relationships between measures of insulin action and metabolic intermediates.

Results

The 75 metabolic intermediates clustered into 19 factors comprising biolo

SUBMITTER: Huffman KM 

PROVIDER: S-EPMC2732163 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature