A gene expression profile for detection of sufficient tumour cells in breast tumour tissue: microarray diagnosis eligibility.

ABSTRACT:

Background

Microarray diagnostics of tumour samples is based on measurement of prognostic and/or predictive gene expression profiles. Typically, diagnostic profiles have been developed using bulk tumour samples with a sufficient amount of tumour cells (usually >50%). Consequentially, a diagnostic results depends on the minimal percentage of tumour cells within a sample. Currently, tumour cell percentage is assessed by conventional histopathological review. However, even for experienced pathologists, such scoring remains subjective and time consuming and can lead to ambiguous results.

Methods

In this study we investigated whether we could use transcriptional activity of a specific set of genes instead of histopathological review to identify samples with sufficient tumour cell

SUBMITTER: Roepman P 

PROVIDER: S-EPMC2732639 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature