G-protein-coupled receptor kinase interacting protein-1 is required for pulmonary vascular development.

ABSTRACT:

Background

The G-protein-coupled receptor kinase interacting protein-1 (GIT1) is a multidomain scaffold protein that participates in many cellular functions including receptor internalization, focal adhesion remodeling, and signaling by both G-protein-coupled receptors and tyrosine kinase receptors. However, there have been no in vivo studies of GIT1 function to date.

Methods and results

To determine essential functions of GIT1 in vivo, we generated a traditional GIT1 knockout mouse. GIT1 knockout mice exhibited approximately 60% perinatal mortality. Pathological examination showed that the major abnormality in GIT1 knockout mice was impaired lung development characterized by markedly reduced numbers of pulmonary blood vessels and increased alveolar spaces. Given that vascul

SUBMITTER: Pang J 

PROVIDER: S-EPMC2732662 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature