Pulsed electron beam water radiolysis for submicrosecond hydroxyl radical protein footprinting.

ABSTRACT: Hydroxyl radical footprinting is a valuable technique for studying protein structure, but care must be taken to ensure that the protein does not unfold during the labeling process due to oxidative damage. Footprinting methods based on submicrosecond laser photolysis of peroxide that complete the labeling process faster than the protein can unfold have been recently described; however, the mere presence of large amounts of hydrogen peroxide can also cause uncontrolled oxidation and minor conformational changes. We have developed a novel method for submicrosecond hydroxyl radical protein footprinting using a pulsed electron beam from a 2 MeV Van de Graaff electron accelerator to generate a high concentration of hydroxyl radicals by radiolysis of water. The amount of oxidation can be controll

SUBMITTER: Watson C 

PROVIDER: S-EPMC2733211 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature