Lectin microarrays identify cell-specific and functionally significant cell surface glycan markers.

ABSTRACT: Glycosylation is among the most complex posttranslational modifications with an extremely high level of diversity that has made it refractory to high-throughput analyses. Despite its resistance to high-throughput techniques, glycosylation is important in many critical cellular processes that necessitate a productive approach to their analysis. To facilitate studies in glycosylation, we developed a high-throughput lectin microarray for defining mammalian cell surface glycan signatures. Using the lectin microarray we established a binary analysis of cell binding and hierarchical organization of 24 mammalian cell lines. The array was also used to document changes in cell surface glycosylation during cell development and differentiation of primary murine immune system cells. To establish the b

SUBMITTER: Tao SC 

PROVIDER: S-EPMC2733773 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature