RAD50 is required for efficient initiation of resection and recombinational repair at random, gamma-induced double-strand break ends.

ABSTRACT: Resection of DNA double-strand break (DSB) ends is generally considered a critical determinant in pathways of DSB repair and genome stability. Unlike for enzymatically induced site-specific DSBs, little is known about processing of random "dirty-ended" DSBs created by DNA damaging agents such as ionizing radiation. Here we present a novel system for monitoring early events in the repair of random DSBs, based on our finding that single-strand tails generated by resection at the ends of large molecules in budding yeast decreases mobility during pulsed field gel electrophoresis (PFGE). We utilized this "PFGE-shift" to follow the fate of both ends of linear molecules generated by a single random DSB in circular chromosomes. Within 10 min after gamma-irradiation of G2/M arrested WT cells, there

SUBMITTER: Westmoreland J 

PROVIDER: S-EPMC2734177 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature