Optically modulated fluorophores for selective fluorescence signal recovery.

ABSTRACT: Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and highly fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation with a secondary laser, enabling high-sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation is demonstrated in high-background environments to extract weak signals from completely obscuring background emission.

SUBMITTER: Richards CI 

PROVIDER: S-EPMC2734916 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature