Parental origin and genome evolution in the allopolyploid Iris versicolor.

ABSTRACT:

Background aims

One of the classic examples of an allopolyploid is Iris versicolor, 'Blue Flag' (2n = 108), first studied by Edgar Anderson and later popularized by George Ledyard Stebbins in cytogenetics and evolutionary text-books. It is revisited here using modern molecular and cytogenetic tools to investigate its putative allopolyploid origin involving progenitors of I. virginica (2n = 70) and I. setosa (2n = 38).

Methods

Genomic in situ hybridization (GISH), fluorescent in situ hybridization (FISH) and Southern hybridization with 5S and 18-26S ribosomal DNA (rDNA) probes were used to identify the parental origin of chromosomes, and to study the unit structure, relative abundance and chromosomal location of rDNA sequences.

Key results

GISH shows that I. versicolor

SUBMITTER: Lim KY 

PROVIDER: S-EPMC2735315 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature