Definitive hematopoiesis initiates through a committed erythromyeloid progenitor in the zebrafish embryo.

ABSTRACT: Shifting sites of blood cell production during development is common across widely divergent phyla. In zebrafish, like other vertebrates, hematopoietic development has been roughly divided into two waves, termed primitive and definitive. Primitive hematopoiesis is characterized by the generation of embryonic erythrocytes in the intermediate cell mass and a distinct population of macrophages that arises from cephalic mesoderm. Based on previous gene expression studies, definitive hematopoiesis has been suggested to begin with the generation of presumptive hematopoietic stem cells (HSCs) along the dorsal aorta that express c-myb and runx1. Here we show, using a combination of gene expression analyses, prospective isolation approaches, transplantation, and in vivo lineage-tracing experiments,

SUBMITTER: Bertrand JY 

PROVIDER: S-EPMC2735398 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature