Ontology highlight
ABSTRACT:
SUBMITTER: Imamura H
PROVIDER: S-EPMC2735558 | biostudies-literature | 2009 Sep
REPOSITORIES: biostudies-literature
Imamura Hiromi H Nhat Kim P Huynh KP Togawa Hiroko H Saito Kenta K Iino Ryota R Kato-Yamada Yasuyuki Y Nagai Takeharu T Noji Hiroyuki H
Proceedings of the National Academy of Sciences of the United States of America 20090831 37
Adenosine 5'-triphosphate (ATP) is the major energy currency of cells and is involved in many cellular processes. However, there is no method for real-time monitoring of ATP levels inside individual living cells. To visualize ATP levels, we generated a series of fluorescence resonance energy transfer (FRET)-based indicators for ATP that were composed of the epsilon subunit of the bacterial F(o)F(1)-ATP synthase sandwiched by the cyan- and yellow-fluorescent proteins. The indicators, named ATeams ...[more]