Solvent signal as an NMR concentration reference.

ABSTRACT: We propose that the NMR solvent signal be utilized as a universal concentration reference because most solvents can be observed by NMR and solvent concentrations can be readily calculated or determined independently. In particular, a highly protonated solvent such as water can serve as a primary concentration standard for its stability, availability, and ease of observation. The potential problems of radiation damping associated with a strong NMR signal can be alleviated by small pulse angle excitation. The solvent signal then can be detected by the NMR receiver with the same efficiency as a dilute analyte. We demonstrated that the analyte's proton concentration can be accurately determined from 4 microM to more than 100 M, referenced by solvent (water) protons of concentrations more than

SUBMITTER: Mo H 

PROVIDER: S-EPMC2735574 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature