Transcriptional profiling of the dose response: a more powerful approach for characterizing drug activities.

ABSTRACT: The dose response curve is the gold standard for measuring the effect of a drug treatment, but is rarely used in genomic scale transcriptional profiling due to perceived obstacles of cost and analysis. One barrier to examining transcriptional dose responses is that existing methods for microarray data analysis can identify patterns, but provide no quantitative pharmacological information. We developed analytical methods that identify transcripts responsive to dose, calculate classical pharmacological parameters such as the EC50, and enable an in-depth analysis of coordinated dose-dependent treatment effects. The approach was applied to a transcriptional profiling study that evaluated four kinase inhibitors (imatinib, nilotinib, dasatinib and PD0325901) across a six-logarithm dose range, us

SUBMITTER: Ji RR 

PROVIDER: S-EPMC2735650 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature